Composite
TG1TLU1

Part:BBa_K3254013:Design

Designed by: Yeqing Zong, Jiayang Li   Group: iGEM19_GENAS_China   (2019-10-16)


Translational Unit for TG1 integrase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1834
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1656
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1870
    Illegal SapI.rc site found at 461
    Illegal SapI.rc site found at 1622


Design Notes

The RiboJ part was added before the RBS-coding sequence, so that the modularity would be improved.


Source

Assembled by this group.

References

Zong, Y., et al., Insulated transcriptional elements enable precise design of genetic circuits. Nat Commun, 2017. 8(1): p. 52.